Spokesman of Indonesian Transport Ministry Bambang Ervan said that the closure began at 05:00 a.m. Jakarta time Saturday (2200 GMT Friday) and will end at 05:00 a.m. Jakarta time Sunday (2200 GMT Saturday).
"Ngurah Rai airport is closed during the day of silence," he told Xinhua by phone.
However, nearby international airport in Lombok keeps operating, said Bambang.
Residents of the Hindu-dominated island are celebrating day of silence. During the sacred day, Bali has shut down internet service, but security, aviation, hospitals and disaster agencies remain operating, according to government officials.
Bali is the center of Indonesian tourism industry with over 400,000 foreign holidaymakers visiting the island every month, according to data from the national statistics bureau.